Morphological characterization of hemocytes in Ectemnaspis rorotaense (Floch & Abonnenc) and Ectemnaspis trombetense (Hamada, Py-Daniel & Adler) (Diptera: Simuliidae)

Juciane Conceição da Silva, Felipe Arley Costa Pessoa, Cláudia Maria Ríos-Velásquez, Helena Rocha Côrrea de Araújo, Ana Paula Sampaio Feitosa, Luiz Carlos Alves, Fábio Andre Brayner, Jansen Fernandes Medeiros

Abstract


Abstract. Hemocytes are insect immune cells which are responsible for processes of phagocytosis, encapsulation, and coagulation. This study aim to characterize the hemocyte cells in two species amazon blackflies: Ectemnaspis rorotaense (Floch & Abonnenc) and Ectemnaspis trombetense (Hamada, Py-Daniel & Adler). Black fly larvae and pupae were collected from streams in Presidente Figueiredo Municipality, Amazonas State, Brazil. Hemolymph of 36 individuals of E. rorotaense (12 larvae, 12 pupae and 12 adults) and 38 of E. trombetense (12 larvae, 12 pupae and 14 adults) were collected and the cells were characterized by light microscopy; 200 adults of each species were used to transmission electron microscopy study. In this work were showed, by the first time, the hemocyte cells of black flies amazon. Four cell types were identified: prohemocytes, granulocytes, oenocytoids, and plasmatocytes. Prohemocytes were the smallest cells and they exhibited a high nuclear-cytoplasmic ratio. Granulocytes possessed large, eccentric nuclei, and they were characterized by the presence of granules that differed in size and shape. Oenocytoids presented poorly developed nucleus with localization in central region. Plasmatocytes showed more morphological variations and large projections in the cytoplasmic membrane. The prohemocytes were the most frequent in E. rorotaense, with nearby 45% of total cells, whereas plasmatocytes and granulocytes, each one with 38%, were the most abundant in E. trombetense. This study showed that prohemocytes, granulocytes, oenocytoids, and plasmatocytes were present in the hemolymph of E. rorotaense and E. trombetense during all stages.

Caracterização Morfológica de Hemócitos em Ectemnaspis rorotaense (Floch & Abonnenc) e Ectemnaspis trombetense (Hamada, Py-Daniel & Adler) (Diptera: Simuliidae)

Resumo. Os hemócitos são células do sistema imune dos insetos responsáveis pelos processos de fagocitose, encapsulação e coagulação. O objetivo desse trabalho foi caracterizar os hemócitos nos simulídeos amazônicos, Ectemnaspis rorotaense (Floch & Abonnenc)e Ectemnaspis trombetense (Hamada, Py-Daniel & Adler). Larvas e pupas de simulídeos foram coletadas em igarapés no município de Presidente Figueiredo, Amazonas, Brasil. Para caracterização celular através da microscopia óptica, foi coletada a hemolinfa de 36 espécimes (12 larvas, 12 pupas e 12 adultos) de E. rorotaense e 38 espécimes (12 larvas, 12 pupas e 14 adultos) de E. trombetense. Para o estudo com microscopia eletrônica de transmissão, 200 adultos de cada espécie foram utilizados. Neste trabalho foram descritos, pela primeira vez, os hemócitos de simulídeos amazônicos. Foram identificados quatro tipos celulares em larvas, pupas e adultos: prohemócitos, plasmatócitos, granulócitos e oenocitóides. Os prohemócitos, com um núcleo volumoso em relação ao citoplasma, se mostraram as menores células. Os granulócitos foram caracterizados pela presença de grânulos de diferentes tamanhos e formas e um núcleo grande e excêntrico. Os oenocitóides revelaram núcleo pouco desenvolvido, geralmente localizado na região central. Os plasmatócitos apresentaram grandes projeções da membrana citoplasmática e maior variação morfológica. Os prohemócitos foram as células mais frequentes em E. rorotaense com 45% do total das células, enquanto os plasmatócitos e granulócitos, ambas com 38% cada, foram as mais abundantes em E. trombetense. Esse estudo mostrou que prohemócitos, granulócitos, oenocitóides e plasmatócitos são presentes na hemolinfa de E. rorotaense and E. trombetense durante todos os estágios.


Keywords


Black fly; cellular immunity; ultrastructure; Imunidade celular; simulídeos; ultra estrutura

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References


Araújo, H.C.R., M.G.S. Cavalcanti, S.S. Santos, L.C. Alves & F.A. Brayner, 2008 Hemocytes ultrastructure of Aedes aegypti (Diptera: Culicidae). Micron, 39: 184-189.

Berger, J. & K. Slavíčková, 2008. Morphological characterization of hemocytes in the adult linden bug, Pyrrhocoris apterus (L.) (Heteroptera). Zoological Studies, 47: 466-472.

Borges, A.R., P.N. Santos, A.F. Furtado & R.C.B.Q. Figueiredo, 2008. Phagocytosis of latex beads and bacteria by hemocytes of the triatomine bug Rhodnius prolixus (Hemiptera: Reduvidae). Micron, 39: 486-494.

Brayner, F.A., H.R.C. Araújo, S.S. Santos, M.G.S. Cavalcanti, L.C. Alves, J.R.B. Souza & C.A. Peixoto, 2007. Haemocyte population and ultrastructural changes during the immune response of the mosquito Culex quinquefasciatus to microfilariae of Wuchereria bancrofti. Medical and Veterinary Entomology, 21: 112-120.

Brayner, F.A., H.R.C. Araújo, M.G.S. Cavalcanti, L.C. Alves & C.A. Peixoto, 2005. Ultrastructural characterization of the hemocytes of Culex quinquefasciatus (Dipetra: Culicidae). Micron, 36: 359-367.

Brayner, F.A., H.R.C. Araújo, S.S. Santos, M.G.S. Cavalcanti, L.C. Alves, J.R.B. Souza & C.A. Peixoto, 2007. Haemocyte population and ultrastructural changes during the immune response of the mosquito Culex quinquefasciatus to microfilariae of Wuchereria bancrofti. Medical and Veterinary Entomology, 21: 112-120.

Bulet, P., C. Hetru, J. Dimarcq & D. Hoffmann, 1999. Antimicrobial peptides in insects structure and function. Developmental & Comparative Immunology, 23: 329-344.

Carneiro, M.E. & E. Daemon, 1996. Caracterização dos tipos celulares presentes na hemolinfa de larvas e ninfas de Rhipicephalus sanguineus (Latreille) (Ixodoidea, Ixodidae) em diferentes estados nutricionais. Revista Brasileira de Zoologia, 13: 609-620.

Castillo, J.C., A.E. Robertson & M.R. Strand, 2006. Characterization of hemocytes from the mosquitoes Anopheles gambiae and Aedes aegypti. Insects Biochemistry and Molecular Biology, 36: 891-903.

Cerqueira, N.L., 1959. Sobre a transmissão da Mansonella ozzardi. Jornal Brasileiro de Medicina 1: 885-914.

Coscarón, S. & C. Coscarón-Arias, 2007. Neotropical simuliidae (Diptera: Insecta), p. 17-18. In: Adis, J., J.R. Arias, G. Rueda-Delgdo & K.M. Wantzen (Eds).: Aquatic biodiversity in Latin America (ABLA). Vol. ensoft, Sofia - Moscow, 685 p.

Cupp, M.S., Y. Chen & E.W. Cupp, 1997. Cellular hemolymph response of Simulium vittatum (Diptera:Simuliidae) to intrathoracic injection of Onchocerca lienalis (Filarioidea: Onchocercidae) microfilariae. Journal of Medical Entomology, 34: 56-63.

Falleiros, A.M.F. & E.A. Gregório, 1995. Hemócitos fagocitários em larvas de Diatraea saccharalis (Fabricius) (Lepidoptera, Pyralidae). Revista Brasileira de Zoologia, 12: 751-758.

Falleiros, A.M.F., M.T.S. Bombonato & E.A. Gregório, 2003. Ultrastructural and quantitative studies of hemocytes in the sugarcane borer, Diatraea saccharalis (Lepidoptera: Pyralidae). Brazilian Archives of Biology and Technology, 46: 287-294.

Gupta, A.P., 1985. Comprehensive Insect Physiology, Biochemistry and Pharmacology, p 401-451. In: Kerkut, G.A. & L.I. Gilbert (Eds), Embryogenesis and Reproduction, vol 1.Oxford: Pergamon Press, 487 p.

Hamada, N. & P.H. Adler, 2001. Bionomia e chave para imaturos e adultos de Simulium (Diptera: Simuliidae) na Amazônia Central, Brasil. Acta Amazônica, 31: 109-132.

Han, S.S. & A.P. Gupta, 1988. Arthropod imune system. V. Activated immunocytes (granulocytes) of the German cockroach. Blattella germanica (L.) (Dictyoptera: Blattellidae) show increased number of microtubules and nuclear pores during immune reacting to foreing tissue. Cell Structure and Function, 13: 333-343.

Hernández-Martinez, S., H. Lanz, H. Rodríguez, J.A. Torres, A. Martínez-Palomo & V. Tsutsumi, 1999. Morphological and cytochemical characterization of female Anopheles albimanus (Diptera: Culicidae) hemocytes. Journal of Medical Entomology, 36: 426-434.

Hillyer, J.F., S.L. Schmidt & B.M. Christensen, 2003. Rapid phagocytosis and melanization of bacteria and Plasmodium sporozoites by hemocytes of the mosquito Aedes aegypti. The Journal of Parasitology, 89: 62-69.

Joshi, P.A. & P.L. Lambdin, 1996. The ultrastructure of hemocytes in Dactylopius confusus (Cockerell), and the role of granulocytes in the synthesis of cochineal dye. Protoplasma, 192: 199-216.

Kuhn, K.H. & T. Haug, 1994. Ultrastructural, cytochemical, and immunocytochemical characterization of haemocytes of the hard tick Ixodes ricinus (Acari; Chelicerata). Cell and tissue research, 3: 493-504.

Lavine, M.D. & M.R. Stand, 2002. Insect hemocytes and their role in immunity. Insect Biochemistry and Molecular Biology, 32: 1295-1309.

Lello, M.D., L.A. Toledo & E.A. Gregório, 1987. Elementos figurados da hemolinfa de Dermatobia hominis (Diptera: Cuterebridae): caracterização ao nível de microscopia óptica, em larvas do 2° e 3° instares. Memórias do Instituto Oswaldo Cruz, 82: 351-358.

Lemaitre, B. & J. Hoffmann, 2007. The host defense of Drosophila melanogaster. Annual Review of Immunology, 25: 697-743.

Luckhart, S., M.S. Cupp & E.W. Cupp, 1992. Morphological and functional classification of the hemocytes of adult female Simulium vittatum (Diptera: Simuliidae). Journal of Medical Entomology, 29: 457-466.

McLaughlin, R.E. & G. Allen, 1965. Description of hemocytes and the coagulation process in the boll weevil, Anthonomus grandis Boheman (Curculionidae). The Biological Bulletim, 128: 112-114.

Pessoa, F.A.C., U.C. Barbosa & J.F. Medeiros, 2008. A new species of Cerqueirellum Py-Daniel, 1983 (Diptera: Simuliidae) and proven new vector of mansonelliasis from the Ituxi River, Amazon basin, Brazil. Acta Amazônica, 38: 560-582.

Py-Daniel, V. & R.T. Moreira-Sampaio, 1994. Jalacingomyia gen. n. (Culicomorpha); a ressurreição de Gymnopaidinae; a eliminação do nível tribal; apresentação de novos caracteres e a redescrição dos estágios larval e pupal de Simulium colombaschense (Fabricius, 1787) (Diptera: Simuliidae). Memorias del Caicet, IV: 101-148.

Shelley, A.J.LC., M. Maia-Herzog, A.D.A. Luna Dias & M.A.P. Moraes, 1997. Biosytematic studies on the Simuliidae (Diptera) of the Amazonia onchocerciasis focus. Bulletin of the British Museum (Natural History (Entomology), 66: 1-121.

Siddiqui, M.I. & M.S. Al-Khalifa, 2012. Circulating Haemocytes in Insects: Phylogenic Review of Their Types. Pakistan Journal of Zoology, 44:1743-1750.

Silva, J.E.B., I.C. Boleli & Z.L.P. Simões, 2002. Hemocyte types and total and differential counts in unparasitized and parasitized Anastrepha obliqua (Diptera, Tephritidae) larvae. Brazilian Journal of Biology, 62: 689-699.

Ribeiro, C., N. Simdes & M.B. Linp, 1996. Insect Immunity: the Haemocytes of the Armyworm Mythimna unipuncta (Lepidoptera: Noctuidae) and their role in defence reactions . In Vivo and In Vitro Studies. Journal of Insects Physiology, 42: 815-822.

Rubtsov, L.A., 1959. Hemolymph and its function in black flies (Diptera: Simuliidade). Entomology Review, 38: 32-50.




DOI: http://dx.doi.org/10.12741/ebrasilis.v8i3.505

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