Morfometria geométrica alar como ferramenta para a identificação de três espécies de califorídeos (Diptera: Calliphoridae) no Distrito Federal.

Marcos Patrício Macedo

Abstract


Resumo.Chrysomya albiceps (Weidemann), Chrysomya megacephala (Fabricius) e Hemilucilia segmentaria (Fabricius)(Diptera, Calliphoridae) estão entre as espécies de dípteros necrófagos mais encontrados em locais de crime no Brasil. A correta identificação do espécime, ou fragmento deste, coletado em um local de crime é etapa fundamental para o emprego da entomologia forense em investigações criminais. Nesse estudo, avaliamos a possibilidade de identificação dessas três espécies de califorídeos pela morfologia alar, por meio de análises de morfometria geométrica. Foram analisadas as asas direitas de 139 espécimes, 55 C. albiceps, 42 C. megacephala e 42 H. segmentaria, por meio de análises de variáveis canônicas e análises discriminantes. Das 278 comparações par a par, apenas 6 seis erros de identificação foram registrados (2,1%), enquanto 22 (7,9%) erros de classificação para o teste de validação cruzada, o que aponta para um alto índice de confiabilidade da técnica. Mais estudos são necessários para a validação dessa técnica para seu uso na prática forense..

Wing geometric morphometry as a tool for the identification of three calliphorid (Diptera: Calliphoridae) species at the Brazilian Cerrado

Abstract. Chrysomya albiceps (Weidemann), Chrysomya megacephala (Fabricius) and Hemilucilia segmentaria (Fabricius) (Diptera: Calliphoridae) are among the fly species of forensic interest commonly found in Brazil. The correct identification of the specimen, or fragment of a specimen, collected at a crime scene is a crucial step for the use of forensic entomology as a tool in criminal investigations. In this study, the discrimination of these three species of Calliphoridae based on wing morphology, by means of geometric morphometric analysis was investigated. Right wings of 139 specimens were analyzed, 55 C. albiceps, 42 C. megacephala e 42 H. segmentaria, through Canonical Variate Analysis, Discriminant Analysis and Cross Validation tests. Of the 278 pairwise comparison, six misidentifications were recorded for discriminant analysis (2.1%), while 22 (7.9 %) misclassification for cross-validation tests, which points to a high technical reliability index. More studies are needed to validate this technique for use in forensic practice.


Keywords


Análise Discriminante; Entomologia Forense; Identificação; Moscas; Taxonomia; Forensic Entomology; Flies; Fly Identification; Discriminant Analysis; Taxonomy

References


Amendt J., C.P. Campobasso, E. Gaudry, C. Reiter, H.N. LeBlanc & M.J.R. Hall, 2007. Best practice in forensic entomology–standards and guidelines. International Journal Legal Medicine, 121: 90-104. DOI: https://doi.org/10.1007/s00414-006-0086-x.

Amendt, J., C.S. Richards, C.P. Campobasso, R. Zehner & M.J.R. Hall, 2011. Forensic entomology: Applications and limitations. Forensic Science, Medicine, and Pathology, 7: 379-392. DOI: https://doi.org/10.1007/s12024-010-9209-2.

Catts, E.P., 1992. Problems in estimating the post-mortem interval in death investigations. Journal Agricultural Entomology, 9: 245-55.

Dujardin, J.P., 2008. Morphometrics applied to medical entomology. Infection, Genetics and Evolution, 8: 875-890. DOI: https://doi.org/10.1016/j.meegid.2008.07.011.

Espra, A.S., S.R.M. Tabugo, M.A.J. Torres, J.G. Gorospe, M.M.E. Manting & C.g. Demayo, 2015. Describing Dimorphism in Wing Shapes in the Blowfly Lucilia Sericata Meigen (Diptera: Calliphoridae) using Geometric Morphometrics. Advances in Environmental Biology, 9: 64-70.

Hall M.J., N. MacLeod & A.H. Wardhana, 2014. Use of wing morphometrics to identify populations of the Old World screwworm fly, Chrysomya bezziana (Diptera: Calliphoridae): a preliminary study of the utility of museum specimens. Acta Tropical, 138 Suppl: S49-55. DOI: https://dx.doi.org.br/10.1016/j.actatropica.2014.03.023.

Kosmann, C., R.P. Mello, E.S. Harterreiten-Souza & J.R. Pujol-Luz, 2013. A List of Current Valid Blow Fly Names (Diptera: Calliphoridae) in the Americas South of Mexico with Key to the Brazilian Species. EntomoBrasilis, 6: 74-85. DOI: https://doi.org/10.12741/ebrasilis.v6i1.266.

Klingenberg, C.P., 2011. MorphoJ: an integrated software package for geometric morphometrics. Molecular Ecology Resources, 11: 353-357. DOI: https://doi.org/10.1111/j.1755-0998.2010.02924.x.

Macedo, M.P., C. Kosmann & J.R. Pujol-luz, 2013. Origin of samples of Cannabis sativa through insect fragments associated with compacted hemp drug in South America. Revista Brasileira de Entomologia, 57: 197-201. DOI: https://doi.org/10.1590/s0085-56262013005000008.

Mello, R.P., 2003. Chave para identificação das formas adultas das espécies da família Calliphoridae (Diptera, Brachycera, Cyclorrhapha) encontradas no Brasil. Entomologia y Vectores, 10: 255-268.

Nuñez J.A. & J. Liria, 2016. Cephalopharyngeal geometric morphometrics in three blowfly species (Diptera: Calliphoridae). Journal of Entomology and Zoology Studies; 4: 338-341.

Pujol-Luz, J.R., L.C. Arantes & R. Constantino, 2008. One hundred years of forensic entomology in Brazil (1908-2008). Revista Brasileira de Entomologia, 52: 485-492.

Ribeiro, P.B. & C.J.B. Carvalho, 1998. Pictorial key to Calliphoridae genera (Diptera) in southern Brazil. Revista Brasileira de Parasitologia Veterinaria, 7: 130-140.

Rohlf, F.J. & L. Marcus, 1993. A Revolution in Morphometrics. Trends Ecology Evolution 8: 129-132.

Rohlf, F.J, 2006. TpsDig, program for Digitizing landmarks and outline for Geometric morphometric analyses, Version 2.04. Depto Ecol. and Evol., Stony Brook, New York. Disponível em: .

Smith K.G.V., 1986. A Manual of Forensic Entomology. British Museum (Natural History), London, and Cornell University Press, Ithaca, NY. 205 p.

Vásquez, M. & J. Liria, 2012. Morfometría geométrica alar para la identificación de Chrysomya albiceps y C. megacephala ( Diptera: Calliphoridae ) de Venezuela. Revista de Biologia Tropical, 60: 1249-1258. DOI: https://doi.org/10.15517/rbt.v60i3.1776.

Wells, J.D. & D.W. Williams, 2007. Validation of a DNA-based method for identifying Chrysomyinae (Diptera: Calliphoridae) used in a death investigation. International Journal of Legal Medicine, 121: 1-8. DOI: https://doi.org/10.1007/s00414-005-0056-8.

Wall, R. & M.L. Warnes, 1994. Responses of the sheep blowfly Lucilia sericata to carrion odour and carbon dioxide. Entomologia experimentalis et applicata 73: 239-246. DOI: https://doi.org/10.1111/j.1570-7458.1994.tb01861.x.

Whitworth, T., 2006. Keys to the genera and species of blow flies (Diptera: Calliphoridae) of America North of Mexico. Proceedings of Entomological Society of Washington, 108: 689-725.




DOI: http://dx.doi.org/10.12741/ebrasilis.v10i1.655

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